摘要：AbstractHydroxyapatite was isolated from the chicken bones of Black Sumatra and Fighter cock by thermal calcinations at 700 °C. The reducing power ofPlumbago indicawas responsible for the green synthesis of silver NPs. The isolated hydroxyapatite was used for the preparation of hydroxyapatite doped silver nanoparticles (HAp/AgNPs) against both Gram-positive and Gram-negative bacteria. The Black Sumatra bone derived HAp/AgNPs were excellent activity againstKlebsiella pneumoniae(28 mm),Staphylococcus aureus(26 mm),Bacillus cereus(24 mm), respectively. Fighting cock bones derived HAp/AgNPs demonstrated activity againstStaphylococcus aureus(22 mm),Klebsiella pneumoniae(25 mm) andBacillus cereus(23 mm), respectively. The MIC value ranged between 45 ± 1.2 µg/mL and 104 ± 0.25 µg/mL. The HAp/AgNPs were characterized by X-ray diffraction (XRD), Fourier Transformed Infrared spectroscopy (FTIR), and Scanning Electron Microscopy (SEM). The XRD patterns of the Hap/Ag revealed functional peaks representing the reflection planes. The HAp/AgNPs showed an ellipsoidal morphology in SEM analysis. The morphological studies at various magnifications indicated that HAp/AgNPs with good crystal structure could prepared by using co-precipitation method.
