摘要：SummaryBlood clot contraction plays an important role in wound healing and hemostasis. Although clot contraction is known to be driven by platelets, how single platelet forces relate to the forces generated by macroscopic clots remains largely unknown. Using our microfabricated high-throughput platelet contraction cytometer, we find that single platelets have an average force of 34 nN (n=10healthy individuals). However, multiple bulk clot experiments predict a mean single platelet force lower than 0.5 nN. To resolve this discrepancy, we use a mesoscale computational model to probe the mechanism by which individual platelets induce forces in macroscopic clots. Our experimentally informed model shows that the number of platelets in the clot cross-section defines the net clot force. We provide a relationship between single platelet force and the clot force that is useful for better understanding of blood disorders associated with bleeding and thrombosis, and facilitates the development of platelet-based and platelet-mimetic biomaterials.Graphical abstractDisplay OmittedHighlights•Single platelet forces are measured using contraction cytometer•Computational model predicts clot contraction force•Clot force is defined by number of platelets in clot cross section•Platelets modulate force depending on mechanical microenvironmentBiological sciences; Biophysics; Biotechnology; Cell biology; Mechanobiology
