标题：A positive feed-forward loop between Fusobacterium nucleatum and ethanol metabolism reprogramming drives laryngeal cancer progression and metastasis
摘要：SummaryAlcohol consumption, which affects the structure and composition of the laryngeal microbiota, is one of the most important risk factors for laryngeal squamous cell cancer (LSCC). Our results demonstrated that high enrichment ofFusobacterium nucleatum(F. nucleatum) in LSCC was associated with poor prognosis.F. nucleatumincreased miR-155-5p and miR-205-5p expression to suppress alcohol dehydrogenase 1B (ADH1B) and transforming growth factor β receptor 2 (TGFBR2) expression by activating innate immune signaling, resulting in ethanol metabolism reprogramming to allowF. nucleatumaccumulation and PI3K/AKT signaling pathway activation to promote epithelial-mesenchymal transition, further exacerbating the uncontrolled progression and metastasis of LSCC. Therefore, the positive feed-forward loop betweenF. nucleatumand ethanol metabolism reprogramming promotes cell proliferation, migration, and invasion to affect LSCC patient prognosis. The amount ofF. nucleatumis a potential prognostic biomarker, which yields valuable insight into clinical management that may improve the oncologic outcome of patients with LSCC.Graphical abstractDisplay OmittedHighlights•Identification ofFusobacterium nucleatumas a risk factor for recurrence in LSCC patients•The positive feed-forward loop betweenF. nucleatumand ethanol metabolism reprogramming•F. nucleatumsuppresses TGFBR2, resulting in EMT in LSCCMicrobial metabolism; Mycology; Cancer
