摘要：SummaryZebrafish regenerate fin rays following amputation through epimorphic regeneration, a process that has been proposed to involve the epithelial-to-mesenchymal transition (EMT). We performed single-cell RNA sequencing (scRNA-seq) to elucidate osteoblastic transcriptional programs during zebrafish caudal fin regeneration. We show that osteoprogenitors are enriched with components associated with EMT and its reverse, mesenchymal-to-epithelial transition (MET), and provide evidence that the EMT markerscdh11andtwist2are co-expressed in dedifferentiating cells at the amputation stump at 1 dpa, and in differentiating osteoblastic cells in the regenerate, the latter of which are enriched in EMT signatures. We also show thatesrp1, a regulator of alternative splicing in epithelial cells that is associated with MET, is expressed in a subset of osteoprogenitors during outgrowth. This study provides a single cell resource for the study of osteoblastic cells during zebrafish fin regeneration, and supports the contribution of MET- and EMT-associated components to this process.Graphical abstractDisplay OmittedHighlights•Osteoblasts express EMT/MET signatures during zebrafish fin regeneration•De/re-differentiating osteoblasts expresscdh11, an EMT marker•A subset of osteoprogenitors express the MET markeresrp1•Our scRNA-seq data can be explored onlineBiological sciences; Developmental biology; Omics; Transcriptomics
