摘要：SummarySymbioses between angiosperms and rhizobia or arbuscular mycorrhizal fungi are controlled through a conserved signaling pathway. Microbe-derived, chitin-based elicitors activate plant cell surface receptors and trigger nuclear calcium oscillations, which are decoded by a calcium/calmodulin-dependent protein kinase (CCaMK) and its target transcription factor interacting protein of DMI3 (IPD3). Genes encoding CCaMK and IPD3 have been lost in multiple non-mycorrhizal plant lineages yet retained among non-mycorrhizal mosses. Here, we demonstrated that the mossPhyscomitriumis equipped with abona fideCCaMK that can functionally complement aMedicagoloss-of-function mutant. Conservation of regulatory phosphosites allowed us to generate predicted hyperactive forms ofPhyscomitriumCCaMK and IPD3. Overexpression of synthetically activated CCaMK or IPD3 inPhyscomitriumled to abscisic acid (ABA) accumulation and ectopic development of brood cells, which are asexual propagules that facilitate escape from local abiotic stresses. We therefore propose a functional role forPhyscomitriumCCaMK-IPD3 in stress-associated developmental reprogrammingGraphical abstractDisplay OmittedHighlights•Conservation of a ‘symbiotic’ calcium-decoding module in a non-mycorrhizal moss•Restoration of symbiosis in legume mutants by complementation with moss homologs•Stress-associated developmental reprogramming by synthetic activation in mossMycology; Developmental biology; Plant biology
