摘要：The in vitro study was designed to evaluate total gas production, dry matter degradability (DMD), and VFA profile; while in vivo study was designed to evaluate nutrient intakes, blood metabolites, and performance of sheep fed native grass mixed with Calliandra calothyrrus (CC), Leucaena leucochepala (LL), Moringa oleifera (MO), Gliricidea sepium (GS), and Artocarpus heterophyllus (AH). The best three from the in vitro results were used to formulate diets in in vivo study. Sixteen male growing sheep (average BW 20 kg) were fed 100% native grass (NG) as control; 70% NG + 30% GS; 70% NG + 30% MO; and 70% NG + 30% AH. Nutrient consumptions, DMD, blood metabolites, and sheep performances were analyzed by using Completely Randomized Design. The in vitro results showed that the total gas production and DMD of CC and LL were the lowest (P<0.05) while the highest was found in GS, MO, and AH treatments (P<0.05). Meanwhile, the in vivo results showed that nutrient intakes (DM, CP, and CF) of GS and AH rations were the highest. The ADG, concentration of albumin, and globulin in all treatments were similar, while total serum protein, triglycerides, and glucose concentration in MO and AH rations were higher than others. Serum cholesterol concentration in MO ration was the lowest, meanwhile the concentration of IgG was the highest (P<0.05). Supplementation of 30% MO was the best choice for optimum rumen fermentation and maintaining health status of local sheep.
其他摘要：Penelitian ini ditujukan untuk mengevaluasi produksi gas total, laju degradasi bahan kering (BK), dan profi l VFA dari Calliandra calothyrrus (CC), Leucaena leucochepala (LL), Moringa oleifera (MO), Gliricidea sepium (GS), dan Artocarpus heterophyllus (AH) in vitro. Kajian in vivo merupakan lanjutan evaluasi in vitro konsumsi nutrien, gambaran metabolit darah, dan performa domba. Sejumlah 16 ekor domba garut jantan (rataan BB 20 kg) diberi ransum kontrol (100% rumput lapang); 70% rumput lapang + 30% GS; 70% rumput lapang + 30% MO; dan 70% rumput lapang + 30% AH dengan rancangan acak lengkap empat perlakuan dan empat ulangan. Hasil in vitro menunjukkan bahwa kandungan tanin dan saponin pada CC cukup tinggi dengan produksi VFA yang rendah. Total produksi gas dan laju degradasi BK pada CC dan LL paling rendah dibandingkan perlakuan lain (P<0,05). Total produksi gas dan laju degradasi BK tertinggi didapat pada GS, MO, dan AH. Konsumsi BK, protein, dan serat kasar pada GS dan AH tertinggi. Laju degradasi BK tertinggi pada MO (P<0,05). Rataan PBBH, serum albumin, dan globulin pada semua perlakuan sama, sedangkan serum protein, tri-gliserida dan glukosa pada perlakuan MO dan AH lebih tinggi daripada perlakuan lain. Perlakuan MO menghasilkan serum kolesterol terendah, sedangkan konsentrasi IgG tertinggi (P<0.05). Suplementasi 30% hijauan MO sangat direkomendasikan karena menghasilkan nilai cerna dengan performa dan status kesehatan yang lebih baik.
