摘要：The research was designed to evaluate chromium incorporation by Ganoderma lucidum in rice straw substrate supplemented with chromium chloride. Treatments were combination of Cr level (0, 500, 1000, 1500, 2000, 25000, and 3000 ppm) and fermentation time (0, 2, 3, and 4 wk). The treatments were arranged in a factorial 7 x 4 and allocated in completely randomized design with three replications. G. lucidum was grown in potato dextrose agar (PDA) medium for 10 days and was inoculated to the substrate which have been sterilized and mixed with CrCl3.6H2O. The moisture of substrate was maintained at 70%. Fiber and protein components of growth media of G. lucidum was determined and analyzed for their Cr content. The finding result showed that addition of Cr up to 3000 ppm into the substrate stimulated the G. lucidum growth. Chromium was incorporated into the fiber and protein components of the growth substrate of G. lucidum during fermentation. Incorporation of Cr into the protein of substrate containing3000 ppm Cr was highest when fermented for 4 wk. Protein component of substrate contained 9.29% Cr while in NDF and ADF was 27.20% and 10.55% Cr, respectively. It is concluded that Cr was incorporated into the G. lucidum cells during fermentation.
其他摘要：Penelitian dirancang untuk mengevalusi inkorporasi kromium oleh fungi Ganoderma lucidum pada substrat jerami padi yang ditambah dengan khromium khlorida, Perlakuan pada penelitian ini adalah kombinasi kadar chromium (0, 500, 1000, 1500, 2000, 2500, dan 3000 ppm) dengan lama fermentasi (0, 2, 3, dan 4 minggu). Perlakuan dirancang dengan menggunakan pola faktorial (7 x 4) yang dialokasikan dalam rancangan acak lengkap dengan tiga ulangan. Starter G. lucidum ditumbuhkan pada media PDA (potato dextrose agar) selama 10 hari dan dinokulasikan pada substrat yang telah ditambah dengan CrCl3 .6H2 O dan diautoclave. Kadar air substrat ditetapkan 70%. Komponen serat dan protein dari media pertumbuhan G. lucidum ditentukan dan dianalisa kandungan Cr-nya. Hasil penelitian menunjukkan bahwa penambahan Cr sampai 3000 ppm pada substrat merangsang pertumbuhan G. lucidum. Kromium terinkorporasi ke dalam komponen serat dan protein pada substrat pertumbuhan G. lucidum. Inkorporasi Cr ke dalam protein substrat yang mengandung 3000 ppm Cr dan difermentasi selama 4 minggu lebih tinggi dibandingkan kadar lainnya. Komponen protein mengandung 9,29% Cr sedangkan di dalam NDF and ADF masing-masing adalah 27,20% dan 10,55% Cr. Dapat disimpulkan bahwa Cr terinkorporasi ke dalam sel miselium G. lucidum selama fermentasi.
