摘要：Cobalt (Co) is required by rumen microorganism for vitamin B12 synthesis. Vitamin B12 is an important cofactor for methionine synthesis and gluconeogenesis. In young ruminants up to 6–8 wk old, the rumen has not been completely developed and rumen microorganisms are not ready to supply vitamin B12. The aim of this research was to determine the potency of mixed rumen bacteria and dietary supplementation of Co and its effect on plasma glucose, blood minerals (Co, Fe, and Zn) concentrations, and hematology of pre-weaning twin lambs. Twelve one month-old local twin lambs were assigned to 4 groups in a randomized complete block design. Lambs were fed cow milk at 10% body weight, adjusted weekly for 80 d. Mixed rumen bacteria were offered at 15 mL/d (8.295x1010 cfu). Dietary treatments were: 1) basal diet (Control), 2) basal diet + 1 mg/kg DM cyanocobalamin (VitB12) and 3) basal diet + 1 mg/kg DM of Co + administration of 15 mL mixed rumen bacteria (CoBac). There were no treatment effects on neither plasma glucose and blood mineral concentrations nor hematological profiles. This study demonstrated that pre-weaning twin lambs are not responsive to supplementation of Co and administration of mixed rumen bacteria.
其他摘要：Kobalt (Co) dibutuhkan mikrob rumen untuk mensintesis vitamin B12. Vitamin B12 berperan dalam sintesis metionin dan glukoneogenesis. Ruminansia berumur di bawah 6-8 minggu belum dapat mensintesis vitamin B12 karena rumen belum berkembang optimal. Tujuan penelitian adalah menggali informasi mengenai potensi campuran bakteri rumen dan Co dalam mengatur glukosa plasma, mineral (Co, Fe, dan Zn) darah, serta hematologi pada anak domba kembar prasapih. Sebanyak 12 ekor anak domba lokal kembar prasapih umur 1 bulan dibagi dalam 4 kelompok dengan rancangan acak kelompok (RAK). Anak domba mendapat susu sapi 10% bobot badan. Campuran bakteri rumen diberikan 15 mL/h (8,295x1010 cfu). Perlakuan terdiri atas: 1) ransum basal (kontrol), 2) ransum basal + suplementasi vitamin B12 sintetis 1 mg/kg BK (VitB12), dan 3) ransum basal + Co 1 mg/kg BK + 15 mL campuran bakteri rumen (CoBac). Glukosa plasma, mineral Co, Fe, dan Zn serta hematologi tidak berbeda antar perlakuan. Pemberian vitamin B12 maupun CoBac tidak mengubah konsentrasi glukosa plasma, status mineral Co, Fe dan Zn serta mempertahankan hematologi normal pada domba kembar prasapih. Penelitian ini memperlihatkan bahwa anak domba kembar prasapih tidak responsif terhadap suplementasi Co dan campuran bakteri rumen.
