摘要：Genetics characterization in livestock based on microsatellite has been widely implemented including for Bali cattle in three different breeding centres in Indonesia. This study aimed to determine diversity of Bali cattle microsatellites in three breeding centers namely BPTU Bali cattle in Bali, BPT-HMT Serading Sumbawa in West Nusa Tenggara, and Village Breeding Center in South Sulawesi. The number of animals used in this study was 95 head of cattle consisted of 32 heads from BPTU Bali Province, 32 heads from BPT-HMT Serading Sumbawa, and 31 heads from Village Barru Breeding Center Barru distric. Microsatellite loci used to determine the diversity was the locus SPS115, INRA037, MM12, and ETH185 based on flourescently labeled fragment method. Data analysis of microsatellite in Bali cattle at three different locations was performed by using POPGEN 1.2, Cervus, and POPTREE2 programs. The results showed that microsatellite diversity in Bali cattle detected 32 alleles from three different locations, and there were specific alleles at each location. Average values of observed heterozygosity (Ho) and expected heterozygosity (He) were 0.418 and 0.604 respectively, while the average value of polymorphism informative content (PIC) was 0.579. The Hardy-Weinberg equilibrium in general suggested that the loci used in the Bali cattle in three populations were in equilibrium, except INRA037 and ETH185. The genetic diversity between populations of Bali cattle was 0.033 (3%), while the inbreeding coefficient index in all populations was 0.296 (29.6 %). Bali cattle phylogeny tree with three populations showed that the populations of Bali cattle in BPTU Bali and VBC Barru had close genetic distance compared to the population of Bali cattle in BPT-HMT Serading Sumbawa. The results of this study provide information that the characteristics of Bali cattle breeding centers in three locations are different, so we need a directed breeding program in each population.
其他摘要：Karakterisasi genetik pada ternak berdasarkan mikrosatelit telah banyak diterapkan termasuk pada sapi Bali di tiga pusat pembibitan di Indonesia. Penelitian ini bertujuan untuk mengevaluasi keragaman mikrosatelit di tiga pusat peternakan BPTU Bali, BPT-HMT Serading Sumbawa di Nusa Tenggara Barat, dan Village Breeding Center Kabupaten Barru di Sulawesi Selatan. Jumlah ternak yang digunakan sebanyak 95 ekor terdiri atas 32 ekor sapi dari BPTU Provinsi Bali, 32 ekor sapi bali dari BPT-HMT Serading Sumbawa, dan 31 ekor sapi Bali dari Village Breeding Center Kabupaten Barru. Lokus mikrosatelit yang digunakan dalam menentukan keragaman adalah SPS115, INRA037, MM12, dan ETH185 berdasarkan metode fragmen flourescently berlabel. Analisis data mikrosatelit pada sapi Bali di tiga lokasi yang berbeda dilakukan menggunakan Program POPGEN1.2, Cervus, dan POPTREE2. Hasil penelitian menunjukkan bahwa keragaman mikrosatelit pada sapi bali menemukan 32 alel dari tiga lokasi yang berbeda dan terdapat alel spesifik di setiap lokasi. Nilai rata-rata yang diamati meliputi nilai heterosigositas (Ho) dan heterozigositas yang diharapkan (He), masing-masing 0,418 dan 0,604, sedangkan nilai rata-rata polymorphism informative content (PIC) adalah 0,579. Keseimbangan Hardy-Weinberg menunjukkan bahwa lokus yang digunakan dalam sapi bali di tiga populasi berada dalam kesetimbangan, kecuali lokus INRA037 dan ETH185. Keragaman genetik antara populasi pada sapi Bali adalah 0,033 (3%), sedangkan laju inbreeding adalah 0,296 (29,6%). Pohon filogenetik di tiga populasi menunjukkan bahwa populasi sapi Bali di BPTU Bali dan VBC Barru memiliki jarak genetik yang dekat dibandingkan dengan populasi sapi Bali di BPT-HMT Serading Sumbawa. Hasil penelitian ini memberikan informasi bahwa karakteristik pusat peternakan sapi bali di tiga pusat peternakan adalah berbeda, sehingga diperlukan program pemuliaan yang terarah di setiap populasi pusat pembibitan sapi bali.
