摘要：The aim of this research was to assess the use of silkworm pupae (Bombyx mori) powder extract on the blood cells and hematological variables of quails (Coturnix coturnix japonica). The treatments consisted of R0= isoenergy and isoprotein ration without pupae powder extract, R1= isoenergy and isoprotein ration + 1% of pupae powder extract, R2= isoenergy and isoprotein ration + 10% of pupae powder extract, R3= isoenergy and isoprotein ration + 1% of residue of pupae powder extract, R4= isoenergy and isoprotein ration + 10% of residue of pupae powder extract, R5= isoenergy and isoprotein ration + 1% of pupae powder, and R6= isoenergy and isoprotein ration + 10% of pupae powder. The variables measured were blood cell and hematological profiles including the number of leukocytes, erythrocytes, and leukocytes differentiation. Immune response was observed at 12, 24, and 48 h by counting the white blood cells type. The results showed that feed consumption ranged 79.19-154.70 g/quail/wk and the body weight was 71.45-149 g/quail. The addition of pupae extract at a dose of 10% in the diet significantly (P<0.05) increased the number of quail white blood cells. The addition of pupae extract, pupae powder residue, and pupae powder in the ration did not significantly affect the number of quail erythrocytes, hemoglobin, and PCV (packed cell volume). Mean number of white blood cells increased during 24 h after treatment of 10% pupae powder extract (R2) and decreased at the 48th h. It is concluded that the value of leukocytes, lymphocytes, monocytes, and heterophile increased in quails supplemented with 10% pupae powder extract (R2).
其他摘要：Tujuan penelitian ini ialah mengkaji pengaruh penggunaan ekstrak tepung pupa ulat sutera (Bombyx mori) pada sel darah dan peubah hematologi puyuh (Coturnix coturnix japonica). Perlakuan terdiri atas R0= ransum isoenergi dan isoprotein tanpa ekstrak tepung pupa, R1= ransum isoenergi dan isoprotein + 1% ekstrak tepung pupa, R2= ransum isoenergi dan isoprotein + 10% ekstrak tepung pupa, R3= ransum isoenergi dan isoprotein + 1% residu ekstrak tepung pupa, R4= ransum isoenergi dan isoprotein + 10% residu ekstrak tepung pupa, R5= ransum isoenergi dan isoprotein + 1% tepung pupa, dan R6= ransum isoenergi dan isoprotein + 10% tepung pupa. Peubah yang diamati adalah profil hematologi meliputi: jumlah leukosit, eritrosit, dan diferensiasi leukosit. Respons kekebalan tubuh diamati pada 12, 24, dan 48 jam setelah pemberian pakan dengan menghitung jenis sel darah putih. Hasil penelitian menunjukkan bahwa konsumsi pakan berkisar 79,19-154,70 g/puyuh/minggu dan bobot badan puyuh memiliki rataan 71,45-149 g. Penambahan ekstrak pupa dosis 10% dalam ransum nyata (P<0,05) meningkatkan jumlah sel darah putih puyuh. Penambahan ekstrak tepung pupa, residu ekstrak tepung pupa, dan tepung pupa dalam ransum tidak berpengaruh pada jumlah eritrosit puyuh, hemoglobin, dan PCV (packed cell volume). Jumlah rata-rata sel darah putih meningkat selama 24 jam setelah penambahan 10% ekstrak tepung pupa (R2) dan menurun pada jam ke-48. Disimpulkan bahwa ada peningkatan nilai leukosit, limfosit, monosit, dan heterofil pada puyuh dengan menambahkan 10% ekstrak tepung pupa (R2).
