摘要：Lactobacillus fermentum strains were successfully isolated from dangke which was a fresh cheese-like product originating from Enrekang, South Sulawesi Province, Indonesia. In addition, Lactobacillus plantarum and Lactobacillus acidophillus were isolated from beef. This study aimed to investigate the ability of those 8 LAB strains from dangke and beef in lowering cholesterol level by using in vitro study. Strain of Lactic acid bacteria used were L. fermentum strains (A323L, B111K, B323K, C113L, C212L), L. plantarum strains (IIA-1A5 and IIA-2C12), and L. acidophillus IIA-2B4. Variables observed were identification of Bile Salt Hydrolase (BSH) gene by Polymerase Chain Reaction (PCR), BSH activity and cholesterol assimilation. Phylogenetic tree indicated homology of L. plantarum IIA-IA5 was 98% to BSH gene of L. plantarum Lp529 with access code of FJ439771 and FJ439775 obtained from GenBank. The results demonstrated that eight strains of LAB isolated from dangke and beef that potentially showed cholesterol-lowering effects were L. fermentum B111K and L. plantarum IIA-1A5. L. fermentum B111K was able to assimilate cholesterol by 4.10% with assimilated cholesterol of 0.13 mg in 1010 cells. In addition, L. plantarum IIA-1A5 had BSH gene and BSH activity, as well as the ability to assimilate cholesterol by 8.10% with assimilated cholesterol of 0.06 mg in 1010 cells. It is concluded that L. fermentum B111K and L. plantarum IIA-1A5 were strains that showed cholesterol-lowering effects.
其他摘要：L. fermentum telah berhasil diisolasi dari dangke yang merupakan sejenis keju segar yang berasal dari Kabupaten Enrekang, Provinsi Sulawesi Selatan, Indonesia, dan L. plantarum dan L. acidophillus yang diisolasi dari daging sapi. Penelitian ini bertujuan untuk mengevaluasi kemampuan 8 strain BAL yang diisolasi dari dangke dan daging sapi dalam menurunkan kolesterol secara in vitro. Strain bakteri yang digunakan adalah L. fermentum (A323L, B111K, B323K, C113L, C212L), strain L. plantarum (IIA-1A5 dan IIA-2C12), dan L. acidophillus IIA-2B4. Peubah yang digunakan ialah uji keberadaan gen Bile Salt Hydrolase (BSH) yang diuji dengan Polymerase Chain Reaction (PCR), aktivitas BSH yang dianalisis secara deskriptif, dan asimilasi kolesterol yang dianalisis menggunakan ANOVA dengan 8 strain dan 3 ulangan. Hasil pohon filogenetik homologi gen BSH L. plantarum IIA-IA5 sebesar 98% dengan L. plantarum Lp529 FJ439771 dan FJ439775 dari GenBank. Hasil penelitian menunjukkan bahwa dari 8 strain BAL isolat dangke dan daging sapi yang diuji, yang berpotensi menurunkan kolesterol adalah L. fermentum B111K dan L. plantarum IIA-1A5. L. fermentum B111K mempunyai kemampuan mengasimilasi kolesterol sebesar 4.10% dengan jumlah kolesterol terasimilasi dalam 1010 sel sebanyak 0.13 mg. L. plantarum IIA-1A5 terbukti memiliki gen BSH yang positif memiliki aktivitas BSH serta mampu mengasimilasi kolesterol sebesar 8.10% dengan jumlah kolesterol terasimilasi dalam 1010 sel sebanyak 0.06 mg. Dapat disimpulkan bahwa strain bakteri yang berpotensi menurunkan kolesterol adalah L. fermentum B111K dan L. plantarum IIA-1A5.
关键词：lactic acid bacteria;bile salt hydrolase;assimilation;cholesterol;dangke
其他关键词：bakteri asam laktat;bile salt hydrolase;asimilasi;kolesterol;dangke
