摘要：AbstractResearchers are now focusing on nanotechnology using metal oxide nanoparticles that have a wide range of antimicrobial activity to treat diseases.Thespesia populneatree belongs to the Malvaceae family grown in tropical areas often found in the mangrove forest. The present study reportedThespesia populneaaqueous bark extract combines with copper metals effectively against skin-infection causing microbes. Qualitative analysis of both aqueous and methanol extract showed the presence of phytochemicals constituents and further conformational by histochemical screening. Quantitative analysis of polyphenol, terpenoids, and triterpenoids was calculated. The synthesized nanoparticles are characterized by several techniques such as UV–Vis spectroscopy, SEM, particle size distribution, EDX, FTIR, and Zeta potential. UV–Vis spectra analysis of the reaction mixture showed the maximum peak was observed at 400 nm. Scanning electron microscopic (SEM) showed the higher density poly-dispersed spherical shaped nanoparticles around 61 to 69 nm in size. Average particle size distribution of the CuONPs is ranged from 60 to 80 nm. Antimicrobial activity of CuONPs is more effective against skin infection-causing microbes of bacterial strains such as (Staphylococcus aureus(MTCC 102),Streptococcus pyogenes(MTCC 102),Pseudomonas aeruginosa(MTCC 358),and fungal strains such asTrichophyton rubrum(MTCC 296),Candida albicans(MTCC 183). The fungal strain ofTrichophyton rubrum(MTCC 296) showed a high zone of inhibition compared with other microbial strains.
