摘要：AbstractObjectivesMycosynthesis offers a benevolent alternative for the synthesis of Silver Nanoparticles (AgNPs) when compared to the conventional physical and chemical methods. Fungi secrete enormous amounts of proteins and enzymes that act as reducing and capping agents in AgNPs synthesis.MethodsAgNPs were synthesized using extracellular fungal extract ofFusarium incarnatum. The reaction mixture was optimized using different parameters to obtain better yield. Anti-melanogenic activity of fungal extract, AgNPs and standard drug (Kojic acid) was evaluated against Human skin melanoma SK-MEL-3 cells. AgNPs were synthesized at 30 °C, pH 5 and 1 mM AgNO3concentration. Then optimization and the characterization were done by UV–Vis, FTIR, XRD, Zeta potential and H-TEM techniques.ResultsThe average size of AgNPs was 10 nm and predominantly spherical in shape. Myco-synthesized AgNPs showed significant anti-melanogenic activity against human skin melanoma SK-MEL-3 cells with an IC50value of 17.70 μg/ml. The concentration of AgNPs required to inhibit SK-MEL-3 cells was lower than the concentrations ofF. incarnatumextract and standard Kojic acid.ConclusionSpherical AgNPs with and average size of 4 nm were synthesized using fungal extract ofF. incarnatum. Mycosynthesized AgNPs can be considered as effective anti-melanogenic agents owing to their impressive activity against skin melanoma.
