摘要：AbstractThe current study focused on anti-biofilm property of chitosan loaded essential oils, which were obtained and evaluated against biofilm formingAcinetobacter baumannii.The results of agar well punctured method demonstrated its effeciency at 150 µg/mL concentration. The complete biofilm arrangement was demolished at 150 µg/mL concentration as suggested by quantitativeinvitroinhibition assay measurement. The biofilm metabolic cells lost their virulence, which was confirmed by color changes of XTT assay. Furthermore, the biofilm metabolic product inactivation was proved by congo red agar assay with the evidence of complete exopolysaccharide arrest. The interior and exterior portions of the chitosan loaded essential oils were completely damaged, as suggested by confocal laser scanning electron microscope and scanning electron microscope observations. Hence, all theinvitroinhibition assays, and morphological examination confirmed that the biofilm formingAcinetobacter baumanniilost its virulence factors and its bacterial growth was eradicated efficiently.
关键词：KeywordsenChitosanEssential oilsBiofilm inhibitionMetabolic activity degradationExopolysaccharide cleavageConfocal laser scanning electron microscope
