摘要：SummaryThe pantry mothPlodia interpunctellais a worldwide pest of stored food products and a promising laboratory model system for lepidopteran functional genomics. Here we describe efficient methods for precise genome editing in this insect. A spontaneous recessive white-eyed phenotype maps to a frameshift deletion (c.737delC) in thewhitegene. CRISPR NHEJ mutagenesis ofwhitereplicates this phenotype with high rates of somatic biallelic knockout. G0individuals with mutant clones on both eyes produced 100% mutant progeny, makingwhitean ideal marker for co-conversion when targeting other genes. CRISPR HDR experiments correctedc.737delCand reverted white eyes to a pigmented state in 37% of G0mosaic adults. These repaired alleles showed practical rates of germline transmission in backcrosses, demonstrating the potential of the technique for precise genome editing.Plodiaoffers a promising avenue for research in this taxon because of its lab-ready features, egg injectability, and editability.Graphical abstractDisplay OmittedHighlights•Plodiapantry moths are an emerging model organism for functional genomics in Lepidoptera•Spontaneous and CRISPR-inducedwhitemutations yield recessive-white eye phenotypes•CRISPR HDR repair with ssODN donor result in practical rates of base editing•We provide optimized protocols forPlodiahandling and genome editingEntomology; Genetic engineering; Genomics; Techniques in genetics
