摘要：SummaryHere, we evaluated the immune properties of the HLA-A2 restricted CD8+T cell epitopes containing mutations from B.1.1.7, and furthermore performed a comprehensive analysis of the SARS-CoV-2 specific CD8+T cell responses from COVID-19 convalescent patients and SARS-CoV-2 vaccinees recognizing the ancestral Wuhan strain compared to B.1.1.7. First, most of the predicted CD8+T cell epitopes showed proper binding with HLA-A2, whereas epitopes from B.1.1.7 had lower binding capability than those from the ancestral strain. In addition, these peptides could effectively induce the activation and cytotoxicity of CD8+T cells. Our results further showed that at least two site mutations in B.1.1.7 resulted in a decrease in CD8+T cell activation and a possible immune evasion, namely A1708D mutation in ORF1ab1707-1716and I2230T mutation in ORF1ab2230-2238. Our current analysis provides information that contributes to the understanding of SARS-CoV-2-specific CD8+T cell responses elicited by infection of mutated strains or vaccination.Graphical abstractDisplay OmittedHighlights•Impaired immune response in CD8+T cells caused by mutant epitopes in B.1.1.7•Identification of the exact epitopes that caused impaired CD8+T cell immune responses•Validation of vaccine still elicited over 60% of the immune protection against B.1.1.7Virology; Immune response
