摘要：SummaryTumor-derived extracellular vesicles (EVs) are involved in tumor metastasis. Highly enriched lncRNA-ALAHM was identified from serum EVs of lung adenocarcinoma (LUAD) patients with liver metastasis by high-throughput sequencing. A mouse model ofin situlung cancer was used to determine the effect of ALAHM in LUAD cell EVs on liver metastasis. The effects of ALAHM on hepatocyte paracrine HGF as well as proliferation, invasion, and migration of LUAD cells were observedin vitro. As results, ALAHM expression in LUAD cell EVs was significantly increased. LUAD-cell-derived EVs overexpressing ALAHM significantly promoted lung cancer liver metastasis in model mice. ALAHM of LUAD cell EVs also promotes hepatocyte parasecretion of HGF by binding with AUF1 and increases the proliferation, invasion, and migration of LUAD cells. Thus, LUAD-cell-derived EVs containing ALAHM causes increasing HGF and promoting liver metastasis of LUAD cells.Graphical abstractDisplay OmittedHighlights•Higher lnc-ALAHM was identified from serum EVs of LUAD patients with liver metastasis•ALAHM of LUAD-cell-derived EVs promotes liver metastasis by increasing HGF•ALAHM of LUAD-cell-derived EVs promotes hepatocyte secretion of HGF by binding AUF1Microenvironment; Molecular biology; Cancer
