摘要：SummaryMelasma is a hyperpigmentary disorder with photoaging features, whose manifestations appear on specific face areas, rich in sebaceous glands (SGs). To explore the SGs possible contribution to the onset, the expression of pro-melanogenic and inflammatory factors from the SZ95 SG cell line exposed to single or repetitive ultraviolet (UVA) radiation was evaluated. UVA up-modulated the long-lasting production of α-MSH, EDN1, b-FGF, SCF, inflammatory cytokines and mediators. Irradiated SZ95 sebocyte conditioned media increased pigmentation in melanocytes and the expression of senescence markers, pro-inflammatory cytokines, and growth factors regulating melanogenesis in fibroblasts cultures. Cocultures experiments with skin explants confirmed the role of sebocytes on melanogenesis promotion. The analysis on sebum collected from melasma patients demonstrated thatin vivosebocytes from lesional areas express the UVA-activated pathways markers observedin vitro. Our results indicate sebocytes as one of the actors in melasma pathogenesis, inducing prolonged skin cell stimulation, contributing to localized dermal aging and hyperpigmentation.Graphical abstractDisplay OmittedHighlights•Irradiated sebocytes produce and upregulate the factors α-MSH, EDN1, SCF. and b-FGF•Sebocyte-derived factors drive modifications of fibroblasts and melanocyte behavior•Sebocytes participate in the skin cells cross-talk regulating pigmentation•Sebocytes locally support the inflammatory and photo-aged environment in melasmaBiological sciences; Biochemistry; Molecular biology
