摘要：Advances in Matrix-assisted Laser Desorption Ionization–time of Flight Mass Spectrometry (MALDI-ToF MS) identification methods have greatly facilitated the rapid identification of yeasts. We validate the use of a shorter on-plate extraction method as well as a lower score cutoff value on the Bruker Biotyper. Overall, when the on-plate extraction method was used together with a score cutoff of > 1.7, a total of 151 of 187 (81%) Candida isolates were correctly identified. At species level, almost 100% of C. krusei and C. albicans isolates were correctly identified. We find the revised protocol to be effective for routine identification of yeasts.
