摘要：The aim of this study was carried out determine the antibiotic susceptibility, heavy metals tolerance and plasmid curing of Shigella species isolating from diarrheal stool samples were collected from different hospitals in El- Dakahlia and Nile River waters, Egypt, from January 2012 to August 2014. After characterization and identification the results obtained show that 172 isolates isolated from stool samples belong to four Shigella species (Shigellasonnei 48.25 %, Shigella flexneri 29.65 %, Shigella dysenteriae 13.95 % and Shigella boydii 8.13 %) while 5 isolates isolated from Nile River water were found belong to (Shigella sonnei 40%, Shigella dysenteriae 40 %and Shigella flexneri 20 %).The antibiotics susceptibility of Shigella sp. to11 antibiotics revealed that the most potent antibiotics were found co-amoxyclav, ciprofloxacin and ceftriaxone respectively while penicillin, ampicillin, co-trimoxazole and chloramphenicol respectively give low activity. The tolerance of Shigella sp. to heavy metals, (cadmium, nickel cobalt and zinc) revealed that all isolates sensitive to 1 and 0.1M concentration. Plasmid profile analysis of ten isolates Shigellasonnei shown that this isolates having numerous plasmid ranged from 8.5 to 4.3 kb. Treat isolates with SDS 2% for 24 hours to plasmid curing after recovery subject to antibiotic sensitivity and heavy metals tolerance. In conclusion, Shigella-associated diarrhea remains relatively common in Egypt and can be used ciprofloxacin and ceftriaxone for treat Shigella sp. infection. The heavy metal tolerance of Shigella sp .associated with resistance to antibiotics ampicillin, tetracycline and chloramphenicol. Present Shigella sp. in Nile River waters indicates polluted with sewage waters and becomes sources of some epidemic diseases.
关键词：Shigella species; antibiotics susceptibility; heavy metals tolerance and plasmid curing
