摘要:This study aimed to investigate the degree of inclusion of 21st century skills - which represents the core of this study-of biology textbook for the ninth grade for the scholastic year 2020/2021.researcher used the analytical descriptive approach that represented content analysis method, The sample Consisted of the Biology textbook for 9th Grade for both semesters the first and the second, A content analysis card was built and improved including the main and sub-skills of 21st century and to verify its psychometric properties validity and reliability. Descriptive statistics through the number of occurrences was used, with their total, percentages, and percentages of agreement between analysts.The results showed that the degree of inclusion for the 21st century main and sub-skills for the 9th Grade Biology textbook in first and second semesters was low, and none of these main or sub-skills have reached the 40% levels. The results showed that the main skill that was mostly included in the book was the critical thinking and problem solving by (38.9%) including the sub-skills. The two parts of the book were permissible from any productive skills and questioning with (0%) results.The skill of making judgments and decisions came in with the highest percentage among the sub-skills, despite the low inclusion of all other sub-skills which have reached only (12%). The results also concluded that there is a clear lack of balance in presenting the twenty-first century skills in the main and sub-skills in the book alike..