摘要：To explore a new and simple rapid extraction and purification technique for phycocyanin, an ionic liquids(ILs)-based aqueous two-phase system(ATPS) was developed for the purification of phycocyanin from Spirulina extracts. Effects of various process parameters such as the concentrations of [Bmim]Cl, the concentrations of KH2PO4, the concentrations of crude phycocyanin, the system pH and the temperature on partitioning of phycocyanin were evaluated. The obtained data indicated that phycocyanin was preferentially partitioned into the ILs-rich phase and the ATPS composed of 23% (w/w) [Bmim]Cland 29% (w/w) KH2PO4 at 30°C and pH 7.0 showed good selectivity on phycocyanin. Under the optimum conditions, phycocyanin with a purity of 3.98 and yield of about 90.23 % was obtained. Therefore, ILs-based ATPS was an effective method for partitioning and recovery of phycocyanin from Spirulina extracts.
