摘要：3-Monochloropropane-1, 2-diol (3-MCPD) is a well-known chloropropanol that is synthesized during food processing contamination. While evaluating sperm quality, sperm penetration into oocytes and early embryonic development, the present study investigated the effects of 3-MCPD onmicereproduction in vitro. During thein vitro fertilization (IVF) process, zygotes and 2-cell embryos were incubated with 3-MCPD-supplemented medium until 8-cell embryo formation to evaluate the reproductive toxicity of 3-MCPD. Our data showed that compared with the control group, the fertilization rate and cleavage rate remarkably decreased in groups treated with 1.802 and 3.160 mM 3-MCPD. The percentage of 2-cell embryo and 4-cell embryo formation also significantly decreased (P < 0.05). Moreover, 8-cell embryos were not observed in any of the treated groups. Taken together, these results suggest that 3-MCPD exposure has hazardous effects on the micesperm fertilization ability as well as embryonic development.
关键词：3-Monochloropropane-1; 2-diol; mouse sperm function; early embryonic development
