摘要:SummarySchistosomiasis is a neglected tropical disease that affects over 200 million people annually. As the antischistosomal drug pipeline is currently empty, repurposing of compound libraries has become a source for accelerating drug development, which demands the implementation of high-throughput and efficient screening strategies. Here, we present a parallelized impedance-based platform for continuous and automated viability evaluation ofSchistosoma mansonischistosomula in 128 microwells during 72 h to identify antischistosomal hitsin vitro. By initially screening 57 repurposed compounds against larvae, five drugs are identified, which reduce parasite viability by more than 70%. The activity profiles of the selected drugs are then investigated via real-time dose-response monitoring, and four compounds reveal high potency and rapid action, which renders them suitable candidates for follow-up tests against adult parasites. The study shows that our device is a reliable tool for real-time drug screening analysis of libraries to identify new promising therapeutics against schistosomiasis.Graphical abstractDisplay OmittedHighlights•Scalable, plastic microwell chip with integrated platinum electrodes•Automated impedance-based recording of 128 microwell units in parallel•Continuous monitoring ofin vitrodrug library efficacy on schistosomula for 72 h•Identification of four fast-acting antischistosomal drugs forin vivotestingBiotechnology; Drugs; Microbiology parasite; Monitoring feature; Signal processing