摘要：SummaryIntestinal intraepithelial lymphocytes (IELs), the first line of defense against microbial and dietary antigens, are classified as natural or induced based on their origin and receptor expression. Induced CD4+CD8αα+TCRβ+T cells (double positive, DPIELs) originated from CD4+CD8α−TCRβ+T cells (single positive, SPIELs) increase with aging. However, the metabolic requirements and the metabolic-related genes in IEL development remain unclear. We determined that the intraepithelial compartment is hypoxic in the presence of microbes and DPIELsincreased more than natural IELs in this location. Moreover, DPIELsconsumed less oxygen and glucose and exhibited unique alterations in mitochondria. Using inhibitors and genetically modified mice, we revealed that DPIELsadapt to their surrounding oxygen-deprived environment in peripheral tissues by modulating specific genes, including hypoxia-inducible factor, mammalian target of rapamycin complexes (mTORC), phosphorylated ribosomal protein S6 (pS6), and other glycolytic factors. Our findings provide valuable insight into the metabolic properties of IELs.Graphical abstractDisplay OmittedHighlights•Microbes induce hypoxic conditions in the intraepithelial compartment•Induced IELs show a lower OCR, glucose uptake, and mitochondrial membrane potential•DPIELsshow reduced expression ofHif1α/Hif2αduring development•Downregulation ofRptorandHif1α/Hif2αexpression in CD4+T cells induces DPIELsBiological sciencesImmunologyComponents of the immune systemCell biology
