摘要：SummaryUpon exposure to the prevailing environment, leaves become increasingly colonized by fungi and bacteria located on the surface (epiphytic) or within (endophytic) the leaves. Many cool season grasses, includingAchnatherum inebrians, host a seed-borne, intercellular, mutualisticEpichloëfungal endophyte, the growth of which is synchronized with the host grass. A study utilizing illumina sequencing was used to examine the epiphytic and endophytic microbial communities inEpichloëendophyte-infected and endophyte-freeA. inebriansplants growing under hot dry field conditions. The presence ofEpichloëendophyte increased the Shannon and decreased Simpson diversity of bacterial and fungal communities.SphingomonasandHymenobacterbacteria andFilobasidiumandMycosphaerellafungi were growing largely epiphytically, whereasMethylobacterium, Escherichia-Shigella,and the fungusBlumeriawere mostly found within leaves with the location of colonization influenced by theEpichloëendophyte. In addition, leaf metabolites inEpichloë-infected andEpichloë-free leaves were examined using LC/MS.Epichloëwas significantly correlated with 132 metabolites.Graphical abstractDisplay OmittedHighlights•Epichloëaltered the composition and diversity of phyllosphere microbial communities•414 detected metabolites were annotated, of which the 132 differential metabolites•There were 229 significant correlations between metabolites and microbial phylaEcology; Mycology; Metabolomics
