摘要：SummaryHybrid hydrogels composed of chitosan (CS) and hyaluronic acid (HA) and collagen (Coll) were prepared by polyelectrolyte complex self-assembly. These scaffolds displayed a good intermingling of the polymeric chains, with porosities above 80% and good interconnected structures with pore sizes lying between 30–115 μm. The ionic interactions between CS and HA make the scaffolds have larger storage modulus and longer LVR regions than their pure counterparts. Both quantities progressively decrease as the HA and Coll concentrations in the formulation rise. These hybrid hydrogels showed good swelling extents from ca. 420 to ca. 690% and suitable resistance to enzymatic degradation, which was slightly lower for scaffolds containing CS to larger extents or Coll in the formulation. All scaffolds were largely cytocompatible and allowed the proliferation of both mouse fibroblast and human keratinocytes with their infiltration inside, thus becoming optimal matrices for intended tissue engineering applications as well as transdermal drug delivery depots.Graphical abstractDisplay OmittedHighlights•Hybrid scaffolds were obtained by polyelectrolyte ionic self-assembly•Scaffolds were largely porous with suitable pore sizes for cell proliferation•Scaffolds showed exceptional swelling and good resistance to enzymatic attack•They were nontoxic and enabled cell proliferation and infiltration inside the scaffoldHealth sciences; Medicine; Materials science; Biomaterials
