标题：Diversity of a Lactic Acid Bacterial Community during Fermentation of Gajami-Sikhae, a Traditional Korean Fermented Fish, as Determined by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
摘要：Gajami-sikhae is a traditional Korean fermented fish food made by naturally fermenting flatfish (
Glyptocephalus stelleri) with other ingredients. This study was the first to investigate the diversity and dynamics of lactic acid bacteria in gajami-sikhae fermented at different temperatures using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). A total of 4824 isolates were isolated from the fermented gajami-sikhae. These findings indicated that
Latilactobacillus,
Lactiplantibacillus,
Levilactobacillus,
Weissella, and
Leuconostoc were the dominant genera during fermentation, while the dominant species were
Latilactobacillus
sakei,
Lactiplantibacillus
plantarum,
Levilactobacillus
brevis,
Weissella
koreensis, and
Leuconostoc
mesenteroides. At all temperatures,
L. sakei was dominant at the early stage of gajami-sikhae fermentation, and it maintained dominance until the later stage of fermentation at low temperatures (5 °C and 10 °C). However,
L.
plantarum and
L.
brevis replaced it at higher temperatures (15 °C and 20 °C). The relative abundance of
L. plantarum and
L. brevis reached 100% at the later fermentation stage at 20 °C. These results suggest that the optimal fermentation temperatures for gajami-sikhae are low rather than high temperatures. This study could allow for the selection of an adjunct culture to control gajami-sikhae fermentation.
关键词：engajami-sikhaeMALDI-TOF MSmicrobial communityculture-dependent methodfermentationidentificationfermentation temperature
