期刊名称：Journal of Advanced Veterinary and Animal Research
印刷版ISSN：2311-7710
出版年度：2022
卷号：9
期号：1
页码：113-121
DOI：10.5455/javar.2022.i575
语种：English
出版社：Bangladesh Agricultural University, Faculty of Veterinary Science
摘要：Objective:
Staphylococcus aureus(
S. aureus) has evolved as one of the most significant bacteria causing food poisoning outbreaks worldwide. This study was carried out to investigate the prevalence, antibiotic sensitivity, virulence, and enterotoxin production of
S. aureus in raw milk of cow from small-scale production units and house-raised animals in Damietta governorate, Egypt.
Material and Methods:
The samples were examined bacteriologically, and antimicrobial sensitivity testing was carried out. Moreover, isolates were characterized by the molecular detection of antimicrobial resistance, virulence, and enterotoxin genes.
Results:
Out of 300 milk samples examined,
S. aureus was isolated from 50 samples (16.7%). Antibiotic sensitivity testing revealed that isolates were resistant to β-lactams (32%), tetracycline (16%), and norfloxacin (16%); however, they showed considerable sensitivity to ceftaroline and amikacin (72%). Multidrug-resistance (MDR) has been observed in eight isolates (16%), with a MDR index (0.5) in all of them. Of the total
S. aureus isolates obtained, methicillin-resistant
S. aureus (MRSA) has been confirmed molecularly in 16/50 (32%) and was found to carry
mecA and
coa genes, while virulence genes;
hlg (11/16, 68.75%) and
tsst (6/16, 37.5%) were amplified at a lower percentage, and they showed a significant moderate negative correlation (
r = −0.59,
p-value > 0.05). Antibiotic resistance genes have been detected in resistant isolates relevant to their phenotypic resistance:
blaZ (100%),
tetK(50%), and
norA (50%). Fifty percent of MRSA isolates carried the
seb enterotoxin gene.
Conclusion:
High detection rate of MRSA and MDR isolates from milk necessitates the prompt implementation of efficient antimicrobial stewardship guidelines, especially at neglected small-scale production units.
