摘要：SummaryThe effects of transcription factors on the maintenance and differentiation of human-induced or embryonic pluripotent stem cells (iPSCs/ESCs) have been well studied. However, the importance of posttranscriptional regulatory mechanisms, which cause the quantitative dissociation of mRNA and protein expression, has not been explored in detail. Here, by combining transcriptome and proteome profiling, we identified 228 posttranscriptionally regulated genes with strict upregulation of the protein level in iPSCs/ESCs. Among them, we found 84 genes were vital for the survival of iPSCs and HDFs, including 20 genes that were specifically necessary for iPSC survival. These 20 proteins were upregulated only in iPSCs/ESCs and not in differentiated cells derived from the three germ layers. Although there are still unknown mechanisms that downregulate protein levels in HDFs, these results reveal that posttranscriptionally regulated genes have a crucial role in iPSC survival.Graphical abstractDisplay OmittedHighlights•The posttranscriptionally regulated 20 genes are necessary for iPSC survival•The proteins of HSPA8, EIF3D, and NCBP2 are quickly degraded in HDFs•mRNA localization affects the protein amounts in most of the 20 genes•Translation is repressed in HDFs despite mRNA binding to ribosomesBiological sciences; Stem cells research; Omics
