摘要：SummaryInsects detect odors via a large variety of odorant receptors (ORs) expressed in olfactory sensory neurons (OSNs). The insect OR is a heteromeric complex composed of a ligand-specific receptor and the co-receptor (ORco). In this study, we identified theORcogene of the cockroach,Periplaneta americana(PameORco), and performed RNAi-based functional analysis ofPameORco. All OSNs in the basiconic sensilla expressedPameORco and received a large variety of odors including sex pheromones. In trichoid sensilla, aPameORco-positive OSN was consistently paired with aPameORco-negative OSN tuned to acids. In adult cockroaches injected withPameORcodsRNA at the nymphal stage, the expression ofPameORco, odor receptions via ORs, and its central processing were strongly suppressed. These results provide new insights into the molecular basis of olfactory reception in the cockroach. The long-lasting and irreversible effects ofPameORcoRNAi would be an effective method for controlling the household pest.Graphical abstractDisplay OmittedHighlights•Whole sequence ofORcoin the American cockroach (PameORco) was characterized•PameORco expressed in olfactory sensory neurons in a sensillar type-specific manner•RNAi chronically and irreversibly suppressed thePameORcoexpression beyond molts•PameORco was essential for receptions of sex pheromones and general odorsEntomology; Molecular neuroscience; Sensory neuroscience
