摘要：SummaryComparative analyses of multiple genomes are used extensively to examine the gains and losses of chemosensory receptors across the genusDrosophila. However, few studies have delved into functional olfactory characteristics. Here we assess olfactory function across 20 species, and identify and describe several similar elements of evolution. We document (a) minor changes in functional ligands based on amino acid substitutions, (b) major changes in olfactory function or perhaps entire receptor replacements, and (c) that only a few receptors are subject to repeated changes, whereas 32 out of 37 OSNs are largely functionally conserved. In addition, we generate a robust model for identifying olfactory function using genomic data and comprehensive ligand-receptor combinations, which includes the prediction of binding pockets. Moreover, this study highlights that functional olfactory evolution does not affect all chemosensory receptors equally, and that ecological, evolutionary, and developmental forces repeatedly affect only a small subset of available receptor proteins.Graphical abstractDisplay OmittedHighlights•We complete an overview ofDrosophila suzukiiolfaction across both the antenna and palps•We examine the evolution of chemosensation across 20 species withinSophophora•We identify sensillum and potential receptor types shifting in their function•Evolution often alters both OR abundance and OR sequence concurrentlyAgricultural science; Entomology; Evolutionary biology; Sensory neuroscience
