摘要:SummaryEarly steps in the endoplasmic reticulum (ER) lumen andcis-Golgi comprise trimming of N-glycans by class I α-mannosidases (MNSs) play crucial roles in root growth and stress response. Herein, we found that the root growth inhibition in themns1 mns2 mns3mutant was partially rescued under alkaline condition, and inhibitor treatment to disrupt auxin transport counteracted this alkaline-maintained root growth. Further study showed that indole-3-acetic acid (IAA) levels were undetectable inmns1 mns2 mns3at normal condition and recovered at alkaline condition, which corroborate our N-glycopeptide profiling, from which N-glycopeptides related with IAA biosynthesis, amino acid conjugates hydrolysis, and response showed differential abundance between normal and alkaline conditions inmns1 mns2 mns3. Overall, our results linked the need for MNSs-mediated N-glycan processing in the ER andcis-Golgi with maintenance of auxin homeostasis and transport inArabidopsisroots during the response to alkaline stress.Graphical abstractDisplay OmittedHighlights•Root growth inhibition of themns1 mns2 mns3mutant was rescued at alkaline pH•Auxin homeostasis was changed between normal and alkaline pH inmns1 mns2 mns3•Disrupting auxin transport inhibited the alkaline-rescued root growth inmns1 mns2 mns3Biological sciences; Plant biology; Plants; Plant physiology