摘要：SummaryGut microbes can synthesize multiple neuro-active metabolites. We profiled neuro-active compounds produced by the gut commensalBacteroides ovatus in vitroandin vivoby LC-MS/MS. We found thatB. ovatusgenerates acetic acid, propionic acid, isobutyric acid, and isovaleric acid.In vitro,B. ovatusconsumed tryptophan and glutamate and synthesized the neuro-active compounds glutamine and GABA. Consistent with our LC-MS/MS-basedin vitrodata, we observed elevated levels of acetic acid, propionic acid, isobutyric acid, and isovaleric acid in the intestines ofB. ovatusmono-associated mice compared with germ-free controls.B. ovatusmono-association also increased the concentrations of intestinal GABA and decreased the concentrations of tryptophan and glutamine compared with germ-free controls. Computational network analysis revealed unique links between SCFAs, neuro-active compounds, and colonization status. These results highlight connections between microbial colonization and intestinal neurotransmitter concentrations, suggesting thatB. ovatusselectively influences the presence of intestinal neurotransmitters.Graphical abstractDisplay OmittedHighlights•Bacteroides ovatusmodulates key neuro-active compoundsin vivoin gnotobiotic animalsNeuroscience; Microbiology; Microbiome
