摘要：SummaryThe social amebaDictyostelium discoideumhas emerged as a powerful model to study mitochondrial genetics and bioenergetics. However, a comprehensive inventory of mitochondrial proteins that is critical to understanding mitochondrial processes has yet to be curated. Here, we utilized high-throughput multiplexed protein quantitation and homology analyses to generate a high-confidence mitochondrial protein compendium consisting of 936 proteins. Our proteomic approach, which utilizes mass spectrometry in combination with mathematical modeling, was validated through mitochondrial targeting sequence prediction and live-cell imaging. Our final compendium consists of 936 proteins. Nearly, a third ofD. discoideummitochondrial proteins do not have homologs in humans, budding yeasts, or an ancestral alphaproteobacteria. Additionally, we leverage our compendium to highlight the complexity of metabolic reprogramming during starvation-induced development. Our compendium lays a foundation to investigate mitochondrial processes that are unique in ameba and to understand the functions of conserved mitochondrial proteins inD. discoideum.Graphical abstractDisplay OmittedHighlights•Identification of 936 high-confidence mitochondrial proteins inD. discoideum•Half of mitochondrial proteins are conserved betweenDictyostelium discoideumand human•Many unique mitochondrial proteins are involved in mtDNA gene expression•Mitochondrial proteins are dynamically expressed during developmentMicrobiology; Microorganism; Omics; Proteomics
