标题：Synthesis and characterization of graphene oxide (GO) sheets for pathogen inhibition: Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa
摘要：AbstractIt is well known that graphene oxide (GO) possesses antibacterial properties towards both Gram-positive and Gram-negative bacteria. As a result, it has recently attracted a lot of interest as an active component towards pathogen inhibition. The aim of this paper is to prepare graphene oxide sheets, with two different oxygen concentrations of 15% and 39%, characterize them, and then investigate in vitro their antimicrobial efficacy in both Gram-positive and Gram-negative bacteria. In particular, GO sheets are synthesized via the Hummer’s method and subsequently, electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy are used to investigate the GO sheet morphology and structure, while Energy Dispersive X-ray Spectroscopy (EDS) is employed for elemental analysis. Subsequently, clinical isolates ofEscherichia coli,Staphylococcus aureusandPseudomonas aeruginosaare incubated with different concentrations of GO sheets, ranging from 0.25 mg/ml to 1.5 mg/ml for 24 h at 37 °C to evaluate the antimicrobial effect. The results confirmed that that Gram positive bacteria are more sensitive to GO, while GO with a higher oxygen content exhibits increased antibacterial activity.
