期刊名称：International Journal of Advances in Engineering and Management
电子版ISSN：2395-5252
出版年度：2021
卷号：3
期号：12
页码：182-200
DOI：10.35629/5252-0311911914
语种：English
出版社：IJAEM JOURNAL
摘要：Cancer is an abnormal condition, which is typified by loss in the control of cellular growth and development leading to intemperate proliferation. In our study, we have used Sarcoma 180, a murine sarcoma cancer cell line. It has been commonly used in research due to it’s rapid growth and it mimics soft tissue sarcoma in humans. This cell line was maintained In vivo in Swiss albino mice via intraperitoneal serial transplantation and development of tumor was monitored by the assessment of several physical parameters like –changes in body weight and survivability of animals after administration of a chemotherapeutic agent. Additionally, cell viability assay of aspirated tumor cells using Trypan blue was performed. Trypan blue is a diazo dye that has been broadly utilized to stain departed tissues or cells on the basis of Dye Exclusion method. This mechanism of trypan blue staining is based on the fact that, it being negatively charged and not interacting with cells unless the membrane is mangled. Thus, all the cells that debar the dye are assessed as operational or living cells while those that takes up the dye are considered non –viable or dead cells. Mitotic index was also performed to calculate the ratio between the number of cells in mitosis and the total number of cells. In this experiment, we have used Vincristine, a vinca alkaloid which acts as a microtubule interfering agent, by blocking metaphase. Tumor bearing mice group were treated with vincristine sulfate to see it’s effects on managing peritoneal ascites. Furthermore, flow cytometric analysis were done to observe the effects of the chemotherapeutic agent, vincristine sulfate to verify our findings.
