期刊名称：International Journal of Advances in Engineering and Management
电子版ISSN：2395-5252
出版年度：2021
卷号：3
期号：7
页码：2863-2873
DOI：10.35629/5252-030726772688
语种：English
出版社：IJAEM JOURNAL
摘要：Vitamin B12 is an essential vitamin which has been used in medical and food industries for years. Since the production of vitamin B12 by chemical synthesis is too expensive and are labor intensive, time consuming and costly, a number of bacterial strains such as Propionibacterium freudenreichii, Propionibacterium shermani and Pseudomonas denitrificans have been successfully used in commercial production of vitamin B 12 industrially. Identification of vitamin B 12 producing microbial strains and synthesis of vitamin B12 from Industrial wastewater especially dairy waste water was the main aim of this study. Isolation, Identification and characterization of Propionibacterium species were carried out using molecular level methods, specifically polymerase chain reaction (PCR). The aim of this research work was to examine the optimization of vitamin B12 production using RSM methodology to produce high yield from Propionibacterium species. Purification of vitamin B12 was carried out by using HPLC method. More recently advances in biotechnology have led to the development of methods that minimize manipulation which provide results in less time and reduce cost.
关键词：vitamin B 12;microbial strains;dairy wastewater;Propionibacterium species;PCR;RSM methodology
