标题：Antibiofilm efficacy of novel biogenic silver nanoparticles from Terminalia catappa against food-borne Listeria monocytogenes ATCC 15,313 and mechanisms investigation in-vivo and in-vitro
摘要：Listeria monocytogenes(LM) is a potential foodborne pathogen, known to form biofilms, which ultimately leads to serious problems in the food industry. This study demonstrates a comprehensive approach for the synthesis of silver nanoparticles using the phytochemicals present in the leaf aqueous extractof Terminalia catappa for the inhibition of L. monocytogens biofilms. The phytochemical analysis by UV–vis spectrum of the extract revealed the presence of polyphenolic compounds such as ellagic acid, gallic acid, chebulinic acid, and chebulgic acid. The shape, structure and size of the synthesized AgNPs were determined as 23–100 nm using the scanning electron microscopic images, and particle size distribution curve analysis. Thein vitrostudies using the AgNPs against L. monocytogenesrevealed the inhibition of biofilm formation, reduction in the Virulence factors such as protease production at sub-inhibitory concentrations of AgNPs.In vivoexperiments performed with theCaenorhabditis elegansmodel showed that AgNPs prolonged the lifespan of infected worms by about 90%. In addition, the non-hazardous nature andin vivoanti-adherence potential of AgNPs were also established with LM -C. elegansinfection model.
