摘要：Enrofloxacin (ENR) is one of the most commonly used antibiotics in pig farms. In this study, using fresh pig manure and corn straw powder as substrates, the effects of different concentrations of ENR (2.5, 10, and 20 mg/L) on anaerobic digestion in completely mixed anaerobic reactors were investigated. A relatively low concentration of ENR (2.5 mg/L) increased methane production by 47.58% compared with the control group. Among the volatile fatty acids (VFAs) in the reactors, the propionic acid content was the lowest, and the concentrations of acetic acid kinase and coenzyme F420 were highest in the first seven days during peak gas production. However, methane production in the reactors with 10 mg/L and 20 mg/L ENR decreased by 8.59% and 20.25%, respectively. Furthermore, the accelerated hydrolysis of extracellular polymeric substances causes a significant accumulation of VFA levels. The microbial community in anaerobic reactors was analyzed by 16S rRNA sequencing. Proteiniphilum was the dominant bacterial genus. In addition, ENR at 2.5 mg/L effectively increased the abundance and diversity of anaerobic microorganisms, whereas a high concentration of ENR (10 and 20 mg/L) significantly decreased these parameters. This study demonstrated that different concentrations of ENR had significantly different effects on anaerobic digestion.
