摘要:To understand ecological and energy problems in the karst area of Guizhou, China, the effects of using biochar-based fertilizers on the energy characteristics of different species of black locust were studied. To determine the most suitable species and the best rational application method of biochar, an outdoor pot experiment was performed using three species of black locust (White-flowered locust (W), Hong-sen locust (S), and Large-leaf fast-growing locust (L)). There were six treatments: control (CK), MF, RH2MF, RH4MF, W2MF, and W4MF (M—compost; F—NPK fertilizer; RH—rice husk biochar; and W—wood biochar), where the numbers represented the mass ratio of biochar to soil. Biochar-based fertilizers had significant effects on the total organic carbon (TOC), total nitrogen (TN), total potassium (TK), branch gross calorific values (GCV), and ash removal calorific values (AFCV) of seedlings. RH4MF had the best overall values. Different species had significant effects in all indicators (except for TN); the effect on S was better than that of W and L. Principal component analysis showed that RH4MF-S had the highest comprehensive scores. In summary, Hong-sen locust (S) was a high-quality energy species and RH4MF may be used as fertilization for energy forest development. This study provides a reference for future long-term energy forest research in this area.