摘要：CpG oligodeoxynucleotides (CpG-ODN) could be expectes to act as an adjuvant for humoral and cellular immunities. The aim of this study was to clarify the mechanism of macrophage activation induced by CpG-ODN. Macrophage activation by CpG-ODN was evaluated by measurement of IL-12 secretion. IL-12 secretion by Cp-ODN was dose-dependent and saturation was observed at 1 μ M . A reactive oxygen species (ROS) scavenger, N -acetylcysteine, inhibited IL-12 secretion, suggesting that the intracellular redox state is important for the activation of macrophages by CpG-ODN. IL-12 secretion was also inhibited by NADPH–oxidase inhibitors, suggesting that ROS generated by NADPH–oxidase is involved in the immunostimulatory effect of CpG-ODN. Nuclear factor (NF)-κB is one of the transcriptional factors involved in IL-12 gene expression, and indeed CpG-ODN activated NF-κB. However, NADPH–oxidase inhibitors did not affect the activation. These results indicated that NADPH–oxidase plays a crucial role in macrophage activation by CpG-ODN and NF-κB does not act as a predominant transcription factor for IL-12 secretion.
