标题：Papyriflavonol A from Broussonetia papyrifera Inhibits the Passive Cutaneous Anaphylaxis Reaction and Has a Secretory Phospholipase A2-Inhibitory Activity
摘要：Papyriflavonol A, a new prenylated flavonol isolated from Broussonetia papyrifera , selectively inhibits recombinant human secretory phospholipase A2s (sPLA2s). Papyriflavonol A was found to inhibit human group IIA and V sPLA2s potently and irreversibly in a dose-dependent manner, with respective IC50 values of 3.9 and 4.5 μ M . The inhibitory effects of papyriflavonol A against bovine group IB (IC50 of 76.9 μ M ) and the human group X (IC50 of 225 μ M ) sPLA2s were weaker than those against human group IIA and V sPLA2s, and human group IIF sPLA2 was not inhibited. In addition, papyriflavonol A potently inhibited the stimulus-induced production of leukotriene C4 with an IC50 value of approximately 0.64 μ M in mouse bone marrow-derived mast cells. In addition, papyriflavonol A significantly reduced IgE-dependent passive cutaneous anaphylaxis in rats. These results indicate that papyriflavonol A provides a basis for novel types of antiinflammatory drugs.
