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  • 标题:Activation of C-Kinase η through Its Cholesterol-3-sulfate-Dependent Phosphorylation by Casein Kinase I in Vitro
  • 本地全文:下载
  • 作者:Maiko Okano ; Takamasa Yokoyama ; Takahiro Miyanaga
  • 期刊名称:Biological and Pharmaceutical Bulletin
  • 印刷版ISSN:0918-6158
  • 电子版ISSN:1347-5215
  • 出版年度:2004
  • 卷号:27
  • 期号:1
  • 页码:109-112
  • DOI:10.1248/bpb.27.109
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:The physiological correlation between casein kinase I (CK-I) and an isoform η of protein kinase C (C-kinase η) was investigated in vitro , since it has been reported that (i) cholesterol-3-sulfate (CH-3S) effectively activates C-kinase η rather than the other isoforms (C-kinase ε and C-kinase δ) in vitro ; and (ii) CK-I efficiently phosphorylates CH-3S-binding proteins, such as high mobility group protein 1 (HMG1), in the presence of CH-3S in vitro . We found that (i) CK-I phosphorylated Thr in preference to Ser on recombinant human C-kinase isoform η (rhC-kinase η) in the presence of CH-3S; (ii) this phosphorylation was selectively inhibited by CK-I-7 (a CK-I inhibitor); and (iii) the activity (phosphorylation of protamine sulfate) of rhC-kinase η was approx. 3.2-fold stimulated by its full phosphorylation by CK-I in the presence of 3 μ M CH-3S. These results suggest that CK-I is a protein kinase responsible for the activation of rhC-kinase η in the presence of CH-3S in vitro .
  • 关键词:casein kinase I;rhC-kinase η;cholesterol-3-sulfate;phosphorylation;C-kinase η activation
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