摘要：β-Dolabrin, γ-thujaplicin, and 4-acetyltropolone, the components of Aomori Hiba ( Thujopsis dolabrata S IEB . et Z UCC . var. hondai M AKINO ), showed antifungal activity on seven kinds of plant-pathogenic fungi, antibacterial activity against two kinds of Legionella sp., and in vitro cytotoxic effect on murine P388 lymphocytic leukemia cell line. Firstly, β-dolabrin, γ-thujaplicin and 4-acetyltropolone had clear antifungal activity against seven kinds of plant-pathogenic fungi tested. In particular, β-dolabrin and 4-acetyltropolone showed strong antifungal activity against Pythium aphanidermatum IFO 32440, with minimum inhibitory concentration (MIC) values of 6.0 μg/ml. Secondly, β-dolabrin, γ-thujaplicin and 4-acetyltropolone had obvious growth-inhibitory effect on two kinds of Legionella sp. 4-Acetyltropolone especially had strong antibacterial activity toward Legionella pneumophila SG 1, and its MIC value was 3.1 μg/ml. These three compounds showed cytotoxic effects against murine P388 lymphocytic leukemia cell line in vitro . The cytotoxic effect of three compounds in the murine P388 lymphocytic leukemia cell line were clear when cell growth was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. At 48 h after treatment, γ-thujaplicin and 4-acetyltropolone at 0.63 μg/ml inhibited cell growth of murine P388 lymphocytic leukemia by 85% and 65%, respectively. At the same time after treatment, the growth of the murine P388 lymphocytic leukemia cell line was completely suppressed by the three compounds at concentrations higher than 5.0 μg/ml. Among these three compounds, γ-thujaplicin had the strongest cytotoxic activity on the growth of this tumor cell line in vitro .
关键词：β-dolabrin;γ-thujaplicin;4-acetyltropolone;antimicrobial activity;cytotoxic activity;murine P388 lymphocytic leukemia cell line
