摘要：In order to develop a new skin whitening agent, safflower ( Carthamus tinctorius L.) seeds were evaluated for melanogenesis inhibitory activity and its active principles were identified following activity-guided isolation. The 80% aqueous methanol extract and ethyl acetate fraction from safflower seeds showed a significant inhibition for mushroom tyrosinase. Three active compounds, N -feruloylserotonin, N -( p -coumaroyl)serotonin, and acacetin, were isolated from the ethyl acetate fraction as the active principles. Compared with arbutin (IC50=0.223 m M ), the IC50 values of these compounds were 0.023, 0.074, and 0.779 m M for N -feruloylserotonin, N -( p -coumaroyl)serotonin, and acacetin, respectively. It was also found that N -feruloylserotonin and N -( p -coumaroyl)serotonin strongly inhibited the melanin production of Streptomyces bikiniensis and B16 melanoma cells in comparison with a known melanogenesis inhibitor, arbutin.
关键词：tyrosinase;melanin;safflower (Carthamus tinctorius L.) seed;skin whitening
