标题：A Role for Corticotropin-Releasing Factor in Repeated Cold Stress-Induced Anxiety-Like Behavior during Forced Swimming and Elevated Plus-Maze Tests in Mice
摘要：SART (specific alternation of rhythm in temperature) stress is known to cause anxiety-like behavior in mice/rats in several anxiety-related behavioral tests. In the present study, we investigated possible roles for corticotropin-releasing factor (CRF) and glucocorticoids in SART stress-induced anxiety-like behavior in two different anxiety-related behavioral tests. In the forced swimming test, CRF, administered intracerebroventricular (i.c.v.) at 0.5—2 pmol/mouse, dose-dependently reduced immobility time in unstressed and SART-stressed mice. α-Helical CRF, a specific CRF receptor antagonist, administered i.c.v. at 0.1—1 nmol/mouse, dose-dependently increased immobility time in SART-stressed mice, but not in unstressed mice. In the elevated plus-maze test, CRF at 10—20 pmol/mouse significantly decreased the time spent in open arms in unstressed mice. CRF at a high dose tended to decrease this time in SART-stressed mice, but this decrease was not statistically significant. α-Helical CRF failed to modify the time in unstressed mice. In contrast, α-helical CRF at 0.38 and 0.75 nmol/mouse increased the time in SART-stressed mice. Both immobility time in the forced swimming test and time spent in open arms in the elevated plus-maze test in unstressed and SART-stressed mice were unaffected by adrenalectomy. These results suggest that CRF plays an important role in anxiety-like behavior caused by SART stress.
关键词：anxiety;repeatedly cold stress;corticotropin-releasing factor (CRF);specific alternation of rhythm in temperature (SART) stress;elevated plus-maze test;forced swimming test
