摘要：Two lignin-degrading basidiomycetes, Stereum hirsutum and Heterobasidium insulare , were used to degrade bisphenol A (BPA) in culture, and the estrogenic activity of the degradation products was examined using MCF-7 cell proliferation assays (E-screen) and analysis of pS2 mRNA expression in MCF 7 cells. Both S. hirsutum and H. insulare showed high resistance to BPA 100 ppm, and their mycelial growth was fully completed within 8 d of incubation at 30 °C. It took 7 to 14 d to achieve complete degradation ( ca. 99%) of BPA by both fungi. MCF-7 cells proliferated actively at a BPA concentration of 10−5 M . However, cell line proliferation was significantly inhibited when the cells were incubated in BPA culture media containing S. hirsutum and H. insulare . Similar results were obtained regarding pS2 mRNA expression. The pS2 mRNA expression levels decreased by 1.5-fold in supernatant from BPA treated with S. hirsutum and H. insulare compared with those treated with BPA alone.
