标题：Inhibitory Effects of Isorhamnetin-3-O-β-D-glucoside from Salicornia herbacea on Rat Lens Aldose Reductase and Sorbitol Accumulation in Streptozotocin-Induced Diabetic Rat Tissues
摘要：The inhibitory effects of compounds from Salicornia herbacea (Chenopodiaceae) on rat lens aldose reductase (RLAR) and sorbitol accumulation in streptozotocin-induced diabetic rat tissues were investigated. The various fractions from the MeOH extract of S. herbacea were tested for their effects on RLAR in vitro . Among them, the EtOAc fraction was found to exhibit a potent RLAR inhibition (IC50=0.75 μg/ml), from which an active principle as a potent AR inhibitor was isolated and its chemical structure was elucidated as isorhamnetin-3- O -β- D -glucoside (1) by spectral analysis. Compound 1 exhibited a potent RLAR inhibition in vitro , its IC50 being 1.4 μ M . Compound 1, when administered orally at 25 mg/kg in streptozotocin (STZ)-induced diabetic rats, caused not only a significant inhibition of serum glucose concentration but also sorbitol accumulation in the lenses, red blood cells (RBC), and sciatic nerves. These results indicate that compound 1 from S. herbacea is a leading compound for further study as a new drug for the prevention and/or treatment of diabetes and its complications.
关键词：Salicornia herbacea;Chenopodiaceae;isorhamnetin-3-O-β- D -glucoside;rat lens aldose reductase;serum glucose concentration;sorbitol accumulation
