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  • 标题:Amino Acid Sequences of Ferredoxins from Atropa belladonna and Hyoscyamus niger: Their Similarities to Those in Other Tropane-Alkaloid-Containing Plants
  • 本地全文:下载
  • 作者:Yoshiki Mino ; Mayumi Yukita ; Nobuhiro Hiratsuka
  • 期刊名称:Biological and Pharmaceutical Bulletin
  • 印刷版ISSN:0918-6158
  • 电子版ISSN:1347-5215
  • 出版年度:2005
  • 卷号:28
  • 期号:8
  • 页码:1535-1538
  • DOI:10.1248/bpb.28.1535
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:The complete amino acid sequences of [2Fe–2S] ferredoxin from Atropa belladonna and Hyoscyamus niger have been determined by automated Edman degradation of the entire S -carboxymethylcysteinyl proteins and of the peptides obtained by enzymatic digestion. These two ferredoxins exhibited 1—8 differences in their amino acid sequences compared to those of other tropane-alkaloid-containing plants ( Scopolia japonica , Datura stramonium , D. metel , and D. arborea ), and only 1 or 4 differences compared to S. japonica and D. arborea . In contrast, 9—23 differences were observed among the other solanaceous ferredoxins. This suggests that tropane-alkaloid-containing plants are closely related taxonomically.
  • 关键词:Atropa belladonna;Hyoscyamus niger;Solanaceae;amino acid sequence;protein chemotaxonomy;ferredoxin
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